The objective of this study was to quantify the antibiotics (oxytetracycline and enrofloxacin) residue levels in raw and layers by RP-HPLC. This study was carried out on one hundred randomly collected raw and layers hen egg samples (20 of each) to evaluate the antibiotic (oxytetracycline and enrofloxacin) residues level qualitatively by high performance liquid chromatography (HPLC). The separation was carried out using mobile phase composed of 0.1%formic acid: Acetonitrile (50:50, v/v) using C18 (Hypersil ODS-BPS, 250 x 4.6mm; 5µ) at a flow rate of 1ml/min. The residues were quantified at detection wave length 350 nm. The obtained results revealed a detectable level of oxytetracycline and enrofloxacin residues which confirm widespread misuses of antibiotics in farms and lack of application of recommended withdrawal times. The boiling effect of broiler hen egg results shows the reduction in concentration of antibiotic residues. These findings recommended that restricted measures and harder regulations must be applied to prevent the misuse residues prior to marketing.
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